|
姓名 |
崔宗强 |
性别 |
男 |
职务 |
|
职称 |
研究员 |
学历 |
博士 |
通讯地址 |
湖北武汉武昌小洪山中区44号中科院武汉病毒所 |
电话 |
027-87199115 |
邮编 |
430071 |
传真 |
027-87199492 |
电子邮件 |
czq@wh.iov.cn |
教育经历
2002/09-2005/07：中国科学院武汉病毒研究所，微生物学专业，博士。
1999/09-2002/07：华中农业大学，微生物学专业，硕士。
1995/09-1999/07：华中农业大学，植保专业，学士。
工作经历
2011/12-至今：中国科学院武汉病毒研究所，病毒学国家重点实验室，病毒纳米生物学学科组长，研究员。
2011/07-2011/12：中国科学院武汉病毒研究所，病毒学国家重点实验室，病毒纳米生物学学科组长。2008/12-2011/06：中国科学院武汉病毒研究所，病毒学国家重点实验室，副研究员。
2005/07-2008/12：中国科学院武汉病毒研究所，助理研究员。
学术兼职
中国生物物理学会纳米生物学分会理事
中国毒理学会纳米毒理专业委员会委员
中国细胞生物学会细胞结构与功能分会委员
中国微生物学会分析微生物学专业委员会委员
中国生物工程学会生物传感、生物芯片与纳米生物技术专业委员会委员
研究内容
开展病毒学与纳米科学的交叉研究：1）以艾滋病毒、流感病毒及新发烈性病毒为对象，发展单病毒示踪等技术，在活细胞与活体水平研究病毒感染分子动态过程和机制，揭示病毒感染致病及宿主应对机理；2）研究功能性病毒纳米组件和系统，发展病毒纳米疫苗与检测等新技术。
主持项目
1. 国家自然科学基金重大研究计划培育项目，91859108，多功能病毒样颗粒用于恶性胶质瘤非侵入性脑内成像和靶向治疗，2019/01-2021/12，70万元，在研，主持。
2. 中国科学院战略性先导科技专项B课题，XDB29050201，病原单颗粒示踪与高灵敏成像，2018/06-2023/06，825万元，在研，主持。
3. 中国科学院前沿科学重点研究项目“青年拔尖科学家”项目，单颗粒示踪技术研究病毒侵染过程与机制，2017/05-2021/12，250万元，在研，主持。
4. 中国科学院青年创新促进会“优秀会员”，2016/01-2018/12，150万元，已结题，主持。
5. 国家自然科学基金重大科研仪器研制项目，21727816，三维超分辨荧光和形貌显微镜的研制，2018/01-2022/12，215.7万元，在研，参加。
6. 国家科技重大专项，2018ZX10301405，病毒单颗粒示踪及高灵敏检测，2018/01-2020/12，600万元，在研，参加。
7. 国家自然科学基金面上项目，31470269，HIV-1病毒脱壳实时成像及机制研究，2015/01-2018/12，85万元，已结题，主持。
8. 中国科学院“生命科学领域优秀青年科技专项”，活细胞内病毒mRNA-蛋白质相互作用可视化研究，2011/01－2013/12，80万元，已结题，主持。
9. 国家重大科学研究计划项目，2011CB933600，量子点标记技术研究病毒侵染过程及宿主应答，2011/01-2015/12，120万元，已结题，参加。
10. 中国科学院战略性先导科技专项子课题，XDA01020403，研究适合自动化的重编程新技术，2011/01-2015/12，320万元，已结题，主持。
11. 中国科学院武汉病毒所“一三五”重大突破项目，生物纳米器件，2012/01-2015/12，280万，已结题，主持。
成果
1. Qin C, Li W, Li Q, Yin W, Zhang X, Zhang Z, Zhang XE, Cui Z*. Real-time dissection of dynamicuncoating of individual influenza viruses. Proc Natl Acad Sci U S A. 2019 Feb12;116(7):2577-2582. doi: 10.1073/pnas.1812632116.
2. Li Q, Yin W, Zhang Z, Zhang X, Zhang XE, Cui Z*. Encapsulating Quantum Dotswithin HIV‑1Virions through Site-Specific Decoration of the Matrix Protein Enables SingleVirus Tracking in Live Primary Macrophages. Nano Lett. 2018, Dec 12;18(12):7457−7468.doi: 10.1021/acs.nanolett.8b02800.
3. Chen M#, Li W#, Zhang Z, Pan J, Sun Y, Zhang X, Zhang XE,Cui Z*. Three-Fragment FluorescenceComplementation for Imaging of Ternary Complexes under Physiological Conditions.Anal Chem. 2018 Nov 20;90(22):13299-13305. doi: 10.1021/acs.analchem.8b02661.
4. Qi Mi, Zhang XE, Sun X, Zhang X, Yao Y, Liu S, Chen Z, LiW, Zhang Z, Chen J*, Cui Z*, IntranasalNanovaccine Confers Homo- and Hetero-Subtypic Influenza Protection. Small. 2018Mar;14(13):e1703207. doi: 10.1002/smll.201703207.
5. Yin W, Li W, Li Q, Qin C, Zhang Z, Zhang X, Zhang XE*, Cui Z*. Structural characterization andanalysis of Human immunodeficiency virus encapsulating quantum dots. J Nanosci Nanotechnol. 2018Jul 1;18(7):4487-4494. doi: 10.1166/jnn.2018.15347.
6. Ma Y#, Wang M#, Li W, Zhang Z, Zhang X, Tan T, Zhang XE*,Cui Z*. Live Cell Imaging of SingleGenomic Loci with Quantum Dot-Labeled TALEs. Nat Commun. 2017 May 8;8:15318.doi:10.1038/ncomms15318.
7. Ma Y#, Wang M#, Li Wei, Zhang Z, Zhang X, Wu G, TanT, Cui Z*, Zhang XE*. Live Visualization ofHIV-1 Proviral DNA Using a Dual-Color-Labeled CRISPR Syetem. Anal Chem. 2017 Dec5;89(23):12896-12901. doi: 10.1021/acs.analchem.7b03584.
8. Fayyadh T#, Ma F#, Qin C, Zhang X, Li W, Zhang XE, ZhangZ, Cui Z*, Simultaneous detection ofmultiple viruses in their co-infected cells using multicolour imaging withself-assembled quantum dot probes, MicrochimicaActa. 2017August, 184(8): 2815-2824, 2017. doi：10.1007/s00604-017-2300-6.
9. Li Q#, Li W#, Yin W, Guo J, Zhang Z, Zeng D, Zhang X, WuY*, Zhang XE*, Cui Z*.Single-Particle Tracking of Human Immunodeficiency Virus Type 1 ProductiveEntry into Human Primary Macrophages. ACS Nano. 2017 Apr 25;11(4):3890-3903.doi: 10.1021/acsnano.7b00275.
10. Zhang X, Qin C, Li W, Zheng Z, Wang H, Cui Z*. Isolation and characterizationof a highly evolved type 3 vaccine-derived poliovirus in China. Virus Res. 2017 Jun 15;238:179-182. doi:10.1016/j.virusres.2017.06.017.
11. Ma Y, He Z, Tan T, Li W, Zhang Z, Song S, Zhang X, Hu Q,Zhou P, Wu Y, Zhang XE*, Cui Z*.Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles.ACS Nano. 2016 Jun 28;10(6):6273-82. doi: 10.1021/acsnano.6b02462.
12. Chen M#, Liu S#, Li W, Zhang Z, Zhang X, Zhang XE*, Cui Z*. Three-Fragment FluorescenceComplementation Coupled with Photoactivated Localization Microscopy forNanoscale Imaging of Ternary Complexes. ACS Nano. 2016 Sep 27;10(9):8482-90.doi: 10.1021/acsnano.6b03543.
13. Sun X, Li W, Zhang X, Qi M, Zhang Z, Zhang XE*, Cui Z*. In Vivo Targeting and Imagingof Atherosclerosis Using Multifunctional Virus-Like Particles of Simian Virus40. Nano Lett. 2016 Oct 12;16(10):6164-6171.
14. Gao D, Lin X, Zhang Z, Li W, Men D, Zhang XE, Cui Z*. Intracellular cargo delivery byvirus capsid protein-based vehicles: From nano to micro. NanomedicineNBM. 2016 Feb;12(2):365-76. doi: 10.1016/j.nano.2015.10.023.
15. Zhang X#, Zhang F#, Ma X, Zhao X, Li W, Zhang Z, Zhang J,Zhang XE*, Cui Z*. Identification ofinteraction between HIV-1 glycoprotein 41 and integrase. Virol Sin. 2016 Oct;31(5):415-424.
16. Zhang X, Zheng Z, Liu X, Shu B, Mao P, Bai B, Hu Q, LuoM, Ma X, Cui Z*, Wang H*. Tick-borneencephalitis virus induces chemokine RANTES expression via activation of IRF-3pathway. J Neuroinflammation. 2016 Aug30;13(1):209. doi: 10.1186/s12974-016-0665-9.
17. Li S, Chen M, Xiong Q, Zhang J, Cui Z*, Ge F*. Characterization of the Translationally ControlledTumor Protein (TCTP) Interactome Reveals Novel Binding Partners in Human CancerCells. J. Proteome Res. 2016 Oct 7;15(10):3741-3751.
18. Ma Y#, Cui Z#,He Z, Li W, Zhang Z, Zhang X, Zhang XE*, Tan T*. Delaying Photobleaching of aLight-Switch Complex for Real-Time Imaging of Single Viral Particle Uncoating.Anal Chem. 2016 Nov 1;88(21):10675-10679.
19. Li Z, Zhang Z, Bi L, CuiZ, Deng J, Wang D *, Zhang XE*. Mutagenesis of mNeptune Red-Shifts EmissionSpectrum to 681-685 nm. PLoS One. 2016 Apr 27;11(4):e0148749. doi:10.1371/journal.pone.0148749.
20. ZhangXE*, CuiZ, WangD. Sensing of biomolecular interactions using fluorescence complementingsystems in living cells. Biosens Bioelectron. 2016Feb 15;76:243-50. doi: 10.1016/j.bios.2015.07.069.
21. Chen M, Li W, Zhang Z, Liu S, Zhang X, Zhang XE*, Cui Z*. Novel near-infrared BiFCsystems from a bacterial phytochrome for imaging protein interactions and drugevaluation under physiological conditions, Biomaterials. 2015 Apr;48:97-107. doi:10.1016/j.biomaterials.2015.01.038.
22. Men D#, Zhang T#, Hou L, Zhou J, Zhang Z, Shi Y, Zhang J,Cui Z, Deng J, Wang D, Zhang XE*,Self-Assembly of Ferritin Nanoparticles into an Enzyme Nano Composite withTunable Size for Ultra-Sensitive Immunoassay, ACS Nano. 2015 Nov 24;9(11):10852-60. doi:10.1021/acsnano.5b03607.
23. Han Y#, Wang S#, Zhang Z, Ma X, Li W, Zhang X, Deng J,Wei H, Li Z, Zhang XE*, Cui Z*. Invivo imaging of protein-protein and RNA-protein interactions using novelfar-red fluorescence complementation systems, Nucleic Acids Res. 2014Jul;42(13):e103. doi: 10.1093/nar/gku408.
24. Li Z, Wang D, Zhang Z, Bi L, Cui Z, Deng J, Zhang XE*. The S28H mutation on mNeptune generatesa brighter near-infrared monomeric, fluorescent protein with improved quantumyield and pH-stability, Acta Biochim Biophys Sin (Shanghai). 2014Sep;46(9):802-9. doi: 10.1093/abbs/gmu063.
25. Yin J, Zhu D, Zhang Z, Wang W, Fan J, Men D, Deng J, WeiH, Zhang X*, Cui Z*, Imaging ofmRNA–Protein Interactions in Live Cells Using Novel mCherry TrimolecularFluorescence Complementation Systems, PLoS One. 2013 Nov 15;8(11):e80851. doi:10.1371/journal.pone.0080851.
26. Gao D, Zhang Z, Li F, Men D, Deng J, Wei H, Zhang XE*, Cui Z*, Quantum dot-induced viralcapsid assembling in dissociation buffer, Int J Nanomedicine. 2013;8:2119-28.doi: 10.2147/IJN.S44534.
27. You X, Zhang Z, Fan J, Cui Z*, Zhang XE*, Functionally Orthologous Viral and CellularMicroRNAs Studied by a Novel Dual-Fluorescent Reporter System, PLoS One. 2012;7(4):e36157. doi:10.1371/journal.pone.0036157.
28. Liu Y, Zhang Z, Zhao X, Wei H, Deng J, Cui Z*, Zhang XE*, Humancytomegalovirus UL94 is a nucleocytoplasmic shuttling protein containing twoNLSs and one NES, Virus Res. 2012 Jun;166(1-2):31-42. doi:10.1016/j.virusres.2012.02.023.
29. Li K, Zhang Z, Luo M, Yu X, Han Y, Wei H, Cui Z*, Zhang XE*, Multifunctionalferritin cage nanostructures for fluorescence and MR imaging of tumor cells, Nanoscale. 2012 Jan 7;4(1):188-93. doi: 10.1039/c1nr11132a.
30. Zhang C#, Gao D#, Zhou G, Chen L, Zhang XE, Cui Z*, He Z*, Label-Free HomogeneousImmunosensor Based on FRET for the Detection of Virus Antibody in Serum, Chem Asian J. 2012 Aug;7(8):1764-7.doi: 10.1002/asia.201200221.
31. Cui Z#, Ren Q#, Wei H, ChenZ, Deng J, Zhang Z, Zhang XE*, Quantum dot–aptamer nanoprobes for recognizingand labeling influenza A virus particles, Nanoscale. 2011 Jun;3(6):2454-7. doi: 10.1039/c1nr10218d.
32. Wang T, Zhang Z, Gao D, Li F, Wei H, Liang X, Cui Z*, Zhang XE, Encapsulation of gold nanoparticlesby simian virus 40 capsids., Nanoscale. 2011Oct 5;3(10):4275-82. doi: 10.1039/c1nr10568j.
33. Liang Y, Zhang Z, Wei H, Hu Q, Deng J, Guo D, Cui Z*, Zhang XE*. Aptamer beacons for visualization ofendogenous protein HIV-1 reverse transcriptase in living cells., Biosens Bioelectron. 2011Oct 15;28(1):270-6. doi: 10.1016/j.bios.2011.07.031.
34. Tian W, Cui Z*,Zhang Z, Wei H, Zhang XE*, Poliovirus 2Apro induces the nucleic translocationof poliovirus 3CD and 3C0 proteins, Acta Biochim Biophys Sin (Shanghai). 2011Jan;43(1):38-44. doi: 10.1093/abbs/gmq112.
35. Han H#, Cui Z#,Wang W, Zhang Z, Wei H, Zhou Y, Zhang XE*, New regulatory mechanisms for theintracellular localization and trafficking of influenza A virus NS1 proteinrevealed by comparative analysis of A/PR/8/34 and A/Sydney/5/97, J Gen Virol. 2010Dec;91(Pt 12):2907-17. doi: 10.1099/vir.0.024943-0.
36. Cui Z, Zhang K, Zhang Z, LiuY, Zhou Y, Wei H, Zhang XE*, Visualization of the dynamic multimerization ofhuman Cytomegalovirus pp65 in punctuate nuclear foci, Virology. 2009 Sep 30;392(2):169-77. doi:10.1016/j.virol.2009.06.021.
37. Liu Y#, Cui Z#,Zhang Z, Wei H, Zhou Y, Wang M, Zhang XE*, The tegument protein UL94 of humancytomegalovirus as a binding partner for tegument protein pp28 identified byintracellular imaging, Virology. 2009 May 25;388(1):68-77. doi:10.1016/j.virol.2009.03.007.
38. Wang W#, Cui Z#,Han H, Zhang Z, Wei H, Zhou Y, Chen Z, Zhang XE*, Imaging and characterizinginfluenza A virus mRNA transport in living cells, Nucleic Acids Res. 2008Sep;36(15):4913-28. doi: 10.1093/nar/gkn475.
39. Li N#, Cui Z#,Zhang Z, Wei H, Zhou Y, Zhang XE*, Live cell imaging of protein interactions inpoliovirus RNA replication complex using fluorescence resonance energy transfer( FRET ), BiochemBiophys Res Commun. 2008 Apr 11;368(3):489-94. doi:10.1016/j.bbrc.2008.01.094.
40. Cui Z, Zhang Z, Zhang XE*,Wen J, Zhou Y, Xie W, Visualizing the dynamic behavior of poliovirusplus-strand RNA in living host cells, Nucleic Acids Res. 2005 Jun7;33(10):3245-52.
奖励
2015年：崔宗强。中国科学院青年创新促进会优秀会员
2010年：崔宗强。中国科学院生命科学领域优秀青年
2009年：崔宗强。中国科学院卢嘉锡青年人才奖
