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蓝柯

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姓名

蓝柯

性别

职务

主任

职称

教授

学历

博士研究生

通讯地址

武汉大学生命科学学院4106

电话

027-68754592

邮编

430072

传真

027-68754592

电子邮件

klan@whu.edu.cn

 

教育经历

1998.09-2001.07     中南大学湘雅医学院,研究生,博士学位

1995.09-1998.06     重庆医科大学,研究生,硕士学位;

1989.09-1994.06     重庆医科大学,本科,学士学位;

 

工作经历

2020.05 - 至今      武汉大学ABSL-III实验室/动物实验中心主任;

2017.01 - 至今      武汉大学病毒学国家重点实验室主任、生命科学学院教授;

2006.08-2016.12     中国科学院上海巴斯德研究所,研究员,教授,博士生导师,肿瘤病毒研究室负责人,病原诊断中心主任;副所长

2002.06-2006.07     美国宾夕法尼亚大学微生物学系,博士后;

2001.08-2002.05     深圳益生堂生物企业有限公司,研发工程师;

1994.07-1995.08     四川省屏山县人民医院,内科住院医师;

 

学术兼职

Journal of Virology》编委(Editorial Board Member)

Journal of Medical Virology》副主编 (Associate Editor)

Viruses》编委(EditorialBoard Member

Science Bulletin》副主编(AssociateEditor

Virologica Sinica》副主编(Associate Editor

17届国际卡波氏肉瘤病毒大会主席

40-44届国际疱疹病毒大会学术委员会委员

美国微生物学会会员

中国微生物学会病毒专业委员会委员

中国病理生理学会肿瘤专业委员会常务委员

湖北省微生物学会副理事长

 

研究内容

致力于肿瘤病毒感染与致瘤的机制研究,利用具有潜伏特征的卡波氏肉瘤相关疱疹病毒(KSHV)以及慢性持续性感染特征的乙型肝炎病毒(HBV)作为模式病原,深入研究病毒和宿主相互作用及感染致病机制,发展有效的抗病毒治疗策略。

 

主持项目

1.国家自然科学基金重点项目(基金号:81930060)

课题名称:卡波氏肉瘤相关疱疹病毒再激活的分子机制研究

执行年限:2020-2024

承担角色:主持人

资助金额:2,970,000人民币

2. 湖北省技术创新项目

课题名称:抗手足口病致病病毒EV71新药的临床前研究(项目编号:2018ACA123

执行年限:2018-2020

承担角色:项目负责人

资助金额:2,000,000人民币

3. 国家重点研发计划(基金号:2016YFA0502100

课题名称: 疱疹病毒感染与致病过程中蛋白质机器的功能机制

执行年限:2017-2021

承担角色:首席科学家

资助金额:32,110,000人民币

4. 法国道达尔基金会国际项目 (基金号:Total2016-4)

课题名称:Development of public health tools for prevention and treatment of

severe hand-foot-mouth disease (HFMD) in children

执行年限:2016-2018

承担角色: 首席科学家

资助金额:1,450,000 欧元

5. 美国国立卫生研究院R01国际项目 (基金号: 1R01AI116442)

课题名称:Versatile functions of LANA in KSHV pathogenesis

执行年限:2015-2019

承担角色: 主持人

资助金额: 520,000美元

6. 国家杰出青年科学基金项目(基金号:81425017

课题名称:KSHV潜伏感染与致瘤机制.

执行年限:2015-2019

承担角色:主持人

资助金额:4,000,000人民币

7. 国际(地区)合作与交流项目NSFC-NIH项目(中美)(基金号:81361120394

课题名称:卡波氏肉瘤相关疱疹病毒(KSHV)基因组在卡波氏肉瘤组织中的表观遗传修饰研究

执行年限:2014-2016

承担角色:主持人

资助金额:1,800,000人民币

成果

1. Liu XJ, Feng JP, Zhang QH, Guo D, Zhang L, Suo T, Hu WJ, Guo M, Wang X, Huang ZX, Xiong Y,  Chen GZ, Chen Y*, Lan K*. Analytical Comparisons of SARS-COV-2 Detection by qRT-PCR and ddPCR With Multiple Primer/Probe Sets. Emerg Microbes Infect. 2020 May 24;1-12.  doi: 10.1080/22221751.2020.1772679.Online ahead of print.

2. Suo T, Liu XJ, Feng JP, Guo M, Hu WJ, Guo D, HafizUllah , Yang Y,  Zhang QH, Wang X, Sajid M, Huang ZX, Deng LP, Chen TL, Liu F, Xu K, Liu Y,  Zhang

 Q, Liu YL, Xiong Y, Chen GZ, Lan K*, Chen Y*. ddPCR:A More Accurate Tool for SARS-CoV-2 Detection in Low Viral Load Specimens.  Emerg Microbes Infect. 2020 May 22;1-30.doi: 10.1080/22221751.2020.1772678.Online ahead of print.

3. Liu Y, Ning Z, Chen Y, Guo M, Liu Y, Gali NK, Sun L, Duan Y, Cai J, Westerdahl D, Liu X, Xu K, Ho KF*, Kan H*, Fu Q*, Lan K*. Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals. Nature. 2020 Apr 27. doi: 10.1038/s41586-020-2271-3.

4. Liu W, Zhang Q, Chen J, Xiang R, Song H, Shu S, Chen L, Liang L, Zhou J, You L, Wu P, Zhang B, Lu Y, Xia L, Huang L, Yang Y, Liu F, Semple MG, Cowling BJ, Lan K*, Sun Z*, Yu H*, Liu Y*. Detection of  Covid-19 in Children in Early January 2020 in Wuhan, China. N Engl J Med. 2020 Apr 2;382(14):1370-1371. doi: 10.1056/NEJMc2003717

5. Xiong Y, Liu Y, Cao L, Wang D, Guo M, Jiang A, Guo D, Hu W, Yang J, Tang Z, Wu H, Lin Y, Zhang M, Zhang Q, Shi M, Liu Y, Zhou Y*, Lan K*, Chen Y*. Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients. Emerg Microbes Infect. 2020 Dec;9(1):761-770. doi: 10.1080/22221751.2020.1747363.

6. Chen L, Liu W, Zhang Q, Xu K, Ye G, Wu W, Sun Z, Liu F, Wu K, Zhong B, Mei Y, Zhang W, Chen Y, Li Y, Shi M, Lan K*, Liu Y*.  RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerg Microbes Infect. 2020 Feb 5;9(1):313-319. doi: 10.1080/22221751.2020.1725399. eCollection 2020.

7.  Wei X, Bai L, Dong L, Liu H, Xing P, Zhou Z, Wu S,Lan, K*,NCOA2 promotes lytic reactivation of Kaposi’s sarcoma-associated herpesvirus by enhancing the expression of the master switch protein RTA. PLoS Pathog 2019 15(11): e1008160.

8.  Tang Q, Li S, Du L, Chen S, Gao J, Cai Y, Xu Z, Zhao, Z, Lan, K*, Wu S*, Emetine protects mice from enterovirus infection by inhibiting viral translation, Antiviral Research 2019, 104650 doi:https://doi.org/10.1016/j.antiviral.2019.104650.

9.  Bai L, Dong J, Liu Z, Rao Y, Feng P*, Lan K*. Viperin catalyzes methionine oxidation to promote protein expression and function of helicases. Sci Adv2019(5): eaax1031

10.  Zhang F, Liang D, Lin X, Zou Z, Sun R,Wang X, Liang X, Kaye KM, Lan K*.NDRG1 facilitates the replication and persistence of Kaposi'ssarcoma-associated herpesvirus by interacting with the DNA polymerase clampPCNA. PLoS Pathog. 2019 Feb27;15(2):e1007628

11.   Yuan F, Gao ZQ, Majerciak V, Bai L, HuML, Lin XX, Zheng ZM*, Dong YH*, Lan K*. The crystal structure of KSHV ORF57 reveals dimeric active sites important for protein stability and function. PLoS Pathog. 2018 Aug 10;14(8):e1007232

12.  Yuan F, Gao ZQ,Majerciak V, Bai L, Hu ML, Lin XX, Zheng ZM, Dong YH, Lan K*. The crystal structureof KSHV ORF57 reveals dimeric active sites important for protein stability andfunction. PLoS Pathog. 2018Aug 10;14(8):e1007232

13.   Li G, ZhuY, Shao D, Chang H, Zhang X, Zhou D, Gao Y, Lan K*, DengQ*. Recombinant covalently closed circular DNA of hepatitis B virus induceslong-term viral persistence with chronic hepatitis in a mouse model. Hepatology.2018 Jan;67(1):56-70.

14.   Wang X, ZouZ, Deng Z, Liang D, Zhou X, Sun R, Lan K*. Male hormones activateEphA2 to facilitate Kaposi's sarcoma-associated herpesvirus infection:Implications for gender disparity in Kaposi's sarcoma. PLoS Pathog.2017 Sep 28;13(9):e1006580.

15.   Zou Z, MengZ, Ma C, Liang D, Sun R, Lan K*. Guanylate-binding protein1 inhibits nuclear delivery of Kaposi's sarcoma-associated herpesvirus virionsby disrupting formation of actin filament. J Virol. 2017Jul 27;91(16). pii: e00632-17

16.   Lin X, SunR, Zhang F, Gao Y, Bin L, Lan K*. The Latency-AssociatedNuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expressionof SUMO/sentrin Specific Peptidase 6 to Facilitate Latency Establishment. JVirol. 2017 Aug 10;91(17). pii: e00806-17

17.   Sun R, TanX, Wang X, Wang X, Yang L, Robertson ES, Lan K*.Epigenetic Landscape of Kaposi's Sarcoma-Associated Herpesvirus Genome inClassic Kaposi's Sarcoma Tissues. PLoS Pathog. 2017 Jan24;13(1):e1006167

18. Li S, Hu H,He Z, Liang D, Sun R, Lan K*.  Fine-Tuning of theKaposi's Sarcoma-Associated Herpesvirus Life Cycle in Neighboring Cells throughthe RTA-JAG1-Notch Pathway. PLoS Pathog. 2016 Oct19;12(10):e1005900

19.  Hu H, Dong J, Liang D, Gao Z, Bai L, Sun R, HuH, Zhang H, Dong Y, Lan K*. Genome-wide mappingof the binding sites and structural analysis of KSHV vIRF2 reveal it is aDNA-binding transcription factor. J Virol. 2015 Nov4;90(3):1158-68

20. Jia B, GuoM, Li G, Yu D, Zhang X, Lan K*, Deng Q*. Hepatitis Bvirus core protein sensitizes hepatocytes to tumor necrosis factor-inducedapoptosis by suppression of the phosphorylation of mitogen-activated proteinkinase kinase 7. J Virol. 2015 Feb 15;89(4):2041-51.

21. Zhao Q,Liang D, Sun R, Jia B, Xia T, Xiao H, Lan K*. Kaposi'ssarcoma-associated herpesvirus-encoded replication and transcription activatorimpairs innate immunity via ubiquitin-mediated degradation of myeloiddifferentiation factor 88. J Virol. 2015 Jan;89(1):415-27

22.  Hu H, Zhang H, Gao Z, Wang D, Liu G, Xu J, Lan K*, Dong Y*. Structure ofthe type VI secretion phospholipase effector Tle1 provides insight into itshydrolysis and membrane targeting. ActaCrystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2175-85

23. Liang D ,Hu H, Li S, Dong J, Wang X, Wang Y, He L, He Z, Gao Y, Gao S, Lan K*.Oncogenic Herpesvirus KSHV Hijacks BMP-Smad1-Id Signaling to PromoteTumorigenesis. PLoS Pathog. 2014 Jul 10;10(7):e1004253.

24. Qi Z, Li G,Hu H, Yang C, Zhang X, Leng Q, Xie Y, Yu D, Zhang X, Gao Y, Lan K*,Deng Q*. Recombinant Covalently Closed Circular Hepatitis B Virus DNA InducesProlonged Viral Persistence in Immunocompetent Mice. J Virol.2014 Jul;88(14):8045-56

25. Sun R,Liang D, Gao Y, Lan K*. Kaposi'sSarcoma-Associated Herpesvirus-Encoded LANA Interacts with Host KAP1 ToFacilitate Establishment of Viral Latency. J Virol. 2014 Jul1;88(13):7331-7344.

26. Han J, WangL, Liu J, Jin M, Hao F, Zhang P, Zhang Z, Wen D, Wu X, Liu G, Ji L, Xu D, ZhouD, Leng Q, Lan K*, Zhang C*. Co-circulation of three HA and two NAsubtypes of avian influenza viruses in Huzhou, China, April 2013: implicationfor the origin of the novel H7N9 virus. J Virol. 2014Jun;88(11):6506-11

27. Wang X, HeZ, Xia T, Li X, Liang D, Lin X, Wen H, Lan K*.Latency-Associated Nuclear Antigen of Kaposi Sarcoma-Associated HerpesvirusPromotes Angiogenesis through Targeting Notch Signaling Effector Hey1. CancerRes. 2014 Apr 1;74(7):2026-37.

28. Wang Z, ZhuK, Bai W, Jia B, Hu H, Zhou D, Zhang X, Zhang X, Xie Y, Bourgine MM, Michel ML,Lan K*, Deng Q*. Adenoviral delivery of recombinanthepatitis B virus expressing foreign antigenic epitopes for immunotherapy ofpersistent viral infection. J Virol. 2014Mar;88(5):3004-15.

29. Han J, JinM, Zhang P, Liu J, Wang L, Wen D, Wu X, Liu G, Zou Y, Lv X, Dong X, Shao B, GuS, Zhou D, Leng Q, Zhang C, Lan K*. Epidemiological linkbetween exposure to poultry and all influenza A(H7N9) confirmed cases in Huzhoucity, China, March to May 2013. Euro Surveill. 2013 May16;18(20). pii: 20481.

30.  Han J, Niu F, Jin M, Wang L, Liu J, Zhang P,Xie B, Wu X, Wen D, Ji L, Liu G, Yang Z, Zhang C, Zhou D, Leng Q, Lan K*. Clinical presentationand sequence analyses of HA and NA antigens of the novel H7N9 viruses. Emerg Microbes Infect. 2013May;2(5):e23.

31. Xia T, LévyL, Levillayer F, Jia B, Li G, Neuveut C, Buendia MA, Lan K*,Wei Y*.The Four and a Half LIM-only Protein 2 (FHL2) Activates TransformingGrowth Factor β (TGF-β)Signaling by Regulating Ubiquitination of the E3 Ligase Arkadia. J BiolChem. 2013 Jan 18;288(3):1785-94.

32. Yang Y, XiaT, Li N, Zhang J, Yang Y, Cong W, Deng Q, Lan K*, ZhouW*. Combined effects of p53 and MDM2 polymorphisms on susceptibility andsurgical prognosis in hepatitis B virus-related hepatocellular carcinoma. ProteinCell. 2013 Jan;4(1):71-81.

33. Lin X, LiX, Liang D, Lan K*. MicroRNAs and unusual small RNAs discovered inKaposi's sarcoma-associated herpesvirusvirions. J Virol. 2012Dec; 86(23): 12717-30.

34. Jin Y, HeZ, Liang D, Zhang Q, Zhang H, Deng Q, Robertson ES, Lan K*.Carboxyl-Terminal Amino Acids 1052 to 1082 of the Latency-Associated NuclearAntigen (LANA) Interact with RBP-Jκ and Are Responsible forLANA-Mediated RTA Repression. J Virol. 2012 May;86(9):4956-69

35. Liang D,Lin X, Lan K*. Looking at Kaposi'sSarcoma-Associated Herpesvirus-Host Interactions from a microRNA Viewpoint.FrontMicrobiol. 2011;2:271.

36. Liu Y, SunR, Lin X, Liang D, Deng Q, Lan K*. Kaposi'ssarcoma-associated herpesvirus-encoded microRNA miR-K12-11 attenuatestransforming growth factor beta signaling through suppression of SMAD5. JVirol. 2012 Feb; 86(3):1372-81.

37. Li X, LiangD, Lin X, Robertson ES, Lan K*. Kaposi's sarcoma-associatedherpesvirus-encoded latency-associated nuclear antigen reduces interleukin-8expression in endothelial cells and impairs neutrophil chemotaxis by degradingnuclear p65. J Virol. 2011 Sep;85(17):8606-15.

38. Qin Y, LiY, Liu W, Tian R, Guo Q, Li S, Li H, Zhang D, Zheng Y, Wu L, Lan K*,WangJ*. Penicilliummarneffei-stimulated dendritic cells enhance HIV-1trans-infection and promote viral infection by activating primary CD4+ T cells.PLoS One. 2011;6(11):e27609

39. Lin X,Liang D, He Z, Deng Q, Robertson ES, Lan K*. miR-K12-7-5p encoded byKaposi's sarcoma-associated herpesvirus stabilizes the latent state bytargeting viral ORF50/RTA. PLoS One. 2011 Jan 20;6(1):e16224.

40. Liang D,Gao Y, Lin X, He Z, Zhao Q, Deng Q, Lan K*. A humanherpesvirusmiRNA attenuates interferon signaling and contributes to maintenanceof viral latency by targeting IKKε. Cell Res.2011 May;21(5):793-806.

41. Wang X, HeB, Zhang Z, Liu T, Wang H, Li X, Zhang Q, Lan K, Lu X,Wen H*. Human herpesvirus-8 in northwestern China: epidemiology andcharacterization among blood donors. Virol J. 2010 Mar 17;7:62.

42. He Z, LiuY, Liang D, Wang Z, Robertson ES, Lan K*. Cellular corepressor TLE2inhibits replication-and-transcription- activator-mediated transactivation andlytic reactivation of Kaposi's sarcoma-associated herpesvirus. J Virol.2010 Feb;84(4):2047-62.

43. Lan K, Murakami M, Bajaj B, Kaul R, He Z, Gan R, FeldmanM, Robertson ES. Inhibition of KSHV-infected primary effusion lymphomas inNOD/SCID mice by gamma-secretase inhibitor. Cancer Biol Ther.2009 Nov;8(22):2136-43.

44. Liu Y, CaoY, Liang D, Gao Y, Xia T, Robertson ES, Lan K*.Kaposi's sarcoma-associated herpesvirus RTA activates the processivity factorORF59 through interaction with RBP-Jkappa and a cis-acting RTA responsiveelement. Virology. 2008 Oct 25;380(2):264-75.

45. Lan K, Verma SC, Murakami M, Bajaj B, Kaul R,Robertson ES. Kaposi's sarcoma herpesvirus-encoded latency-associated nuclearantigen stabilizes intracellular activated Notch by targeting the Sel10protein. Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16287-92.

46. Lan K, Murakami M, Choudhuri T, Tsai DE, SchusterSJ, Wasik MA, and Robertson E. Detection of Epstein-Barr Virus in T-CellProlymphocytic Leukemia cells in vitro. J Clin Virol. 2008;43:260-5.

47. Verma S, Lan K,Choudhuri T, Cotter MA, and Robertson E. An autonomous replicating elementwithin the KSHV genome. Cell Host Microbe. 2007Aug 16;2(2):106-18.

48. Lan K, Choudhuri T, Murakami M, Kuppers DA, andRobertson E. Intracellular Activated Notch1 Is Critical for Proliferation ofKSHV Associated B Lymphoma Cell Lines in vitro. JVirol. 2006, 80(13):6411-6419

49. Lan K, Murakami M, Choudhuri T, Kuppers D, andRobertson E. Intracellular Activated Notch1 Can Reactivate KSHV from Latency.Virology.2006, 351:393-403.

50. Lan K, Kuppers D, Verma S, Sharma N, Murakami M,and Robertson E. Induction of KSHV Latency Associated Nuclear Antigen by theLytic Transactivator RTA: A Novel Mechanism for Establishment of Latency. JVirol. 2005;79(12):7453-65

51. Lan K, Kuppers D, and Robertson E. KSHVReactivation is Regulated by Interaction of LANA with RBP-Jk, the MajorDownstream Effector of the Notch Signaling Pathway. J Virol. 2005;79(6):3468-78

52. Lan K, Kuppers DA, Verma SC, and Robertson E.Kaposi’s Sarcoma-Associated Herpesvirus-Encoded Latency-Associated NuclearAntigen Inhibits Lytic Replication by Targeting Rta: a Potential Mechanism forVirus-Mediated Control of Latency. J Virol. 2004;78:6585-6594

 

奖励

2016 国家万人计划领军人才

2016 中科院特聘研究员

2015 上海市先进工作者

2015 上海领军人才

2014 国家中青年科技创新领军人才

2014 国家杰出青年科学基金获得者

2012 药明康德生命化学研究奖

2012 上海市黄浦区十大自主创新领军人才称号

2011 国务院政府特殊津贴

2011 中科院朱李月华优秀教师奖

2010 上海市创先争优世博先锋“五带头”优秀共产党员称号

2010 明治乳业生命科学奖

2009 新世纪百千万人才工程国家级人选

2008 上海市浦江人才

2006 美国白血病和淋巴瘤学会Special Fellow Award

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